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The document details a laboratory procedure for extracting genomic DNA from an unknown insect using a silica-based spin column extraction kit, followed by quality evaluation through agarose gel electrophoresis. It also describes the amplification of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene using PCR, including the preparation of a master mix and the thermocycling process. The results of the PCR amplification were again analyzed using agarose gel electrophoresis to confirm the success of

Author

Lucia Flores

Language

EN