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DNAzyme motor systems and logic gates facilitated by toehold exchange translators by Wenchan Deng; Jing Yang Xu; Hanyong Peng; Cheng Zhi Huang; X. Chris Le; Hongquan Zhang is a Engineering article available to read on EtoBox.

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DNAzyme motor systems using gold nanoparticles (AuNPs) as scaffolds are useful for biosensing and in situ amplification because these systems are free of protein enzymes, isothermal, homogeneous, and sensitive. However, detecting different targets using the available DNAzyme motor techniques requires redesigns of the DNAzyme motor. We report here a toehold-exchange translator and the translator-mediated DNAzyme motor systems, which enable sensitive responses to various nucleic acid targets using the same DNAzyme motor without requiring redesign. The translator is able to efficiently convert different nucleic acid targets into a specific output DNA that further activates the pre-silenced DNAzyme motor and consequently initiates the autonomous walking of the DNAzyme motor. Simply adjusting the target-binding region of the translator enables the same DNAzyme motor system to respond to various nucleic acid targets. The translator-mediated DNAzyme motor system is able to detect as low as 2.5 pM microRNA-10b and microRNA-21 under room temperature without the need of separation or washing. We further demonstrate the versatility of the translator and the DNAzyme motor by successful constru

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Author

Wenchan Deng; Jing Yang Xu; Hanyong Peng; Cheng Zhi Huang; X. Chris Le; Hongquan Zhang

Publisher

Elsevier BV

Published

2022

Language

EN

Field

Engineering (Physical Sciences)