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Adenine nucleotide metabolism by chondrocytes in vitro: Role of ATP in chondrocyte maturation and matrix mineralization by Masashi Hatori; Cristina C. Teixeira; Kristine Debolt; Maurizio Pacifici; Irving M. Shapiro is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The objective of the investigation was to explore the notion that chondrocytes in the growth plate secrete nucleotides and that these compounds are used to regulate cell maturation and matrix mineralization. Chondrocytes were isolated from the cephalic region of chick embryo sterna and maintained in culture until confluent. To promote expression of the mature phenotype, cultures were then treated with retinoic acid. During the culture period, medium was removed and analyzed for nucleotides using a modified reverse‐phase high‐performance liquid chromatography (HPLC) procedure. We found that culture medium, conditioned by the chondrocytes, contained significant quantities of nucleotides. Moreover, the nucleotide concentrations were similar in magnitude to levels reported for media conditioned by other cell types. In terms of species, adenosine diphosphate (ADP) was the major nucleotide present in the conditioned medium; adenosine monophosphate (AMP) was present, but at a lower concentration than ADP. To examine the possibility that adenosine triphosphate (ATP) was released by the cultured chondrocytes, but was rapidly degraded into ADP and AMP, we examined the kinetics of

Who reads Adenine nucleotide metabolism by chondrocytes in vitro: Role of ATP in chondrocyte maturation and matrix mineralization?

Author

Masashi Hatori; Cristina C. Teixeira; Kristine Debolt; Maurizio Pacifici; Irving M. Shapiro

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1995

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)