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Incorporation of Boronic Acid into Aptamer-Based Molecularly Imprinted Hydrogels for Highly Specific Recognition of Adenosine by Yuqing Li; Zijie Zhang; Biwu Liu; Juewen Liu is a Materials Science article available to read on EtoBox.

What is Incorporation of Boronic Acid into Aptamer-Based Molecularly Imprinted Hydrogels for Highly Specific Recognition of Adenosine about?

Molecularly imprinted polymers (MIPs) and aptamers are two types of molecular recognition strategies. We are interested in combining them to further improve specificity. Adenosine is a model analyte for developing aptamer-based biosensors, but the most commonly used DNA aptamer for adenosine can also bind deoxyadenosine with a similar affinity ( (adenosine)/ (deoxyadenosine) = 1.07). Since adenosine and deoxyadenosine differ by the former having a -diol, and boronic acid can bind -diol specifically, in this work, an acrydite-modified adenosine aptamer was copolymerized with a boronic acid containing monomer, 3-acrylamidophenylboronic acid (AAPBA) in the presence of adenosine as the template for imprinting. Isothermal titration calorimetry (ITC) and SYBR Green I staining were used to measure its binding. The AAPBA-containing aptamer-MIP exhibited a 115-fold high selectivity for adenosine against deoxyadenosine at pH 6.4, and 230-fold for adenosine against cytidine. We recently found that boronic acid containing hydrogels can nonspecifically adsorb DNA oligonucleotides and inhibit aptamer binding. The ribose in adenosine may interact with the boronic acid unit and decrease its inhibi

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Author

Yuqing Li; Zijie Zhang; Biwu Liu; Juewen Liu

Published

2019

Language

EN

Field

Materials Science (Physical Sciences)