Can I read Mutational analysis of loxP sites for efficient Cre-mediated insertion into genomic DNA on EtoBox?

Mutational analysis of loxP sites for efficient Cre-mediated insertion into genomic DNA by James G. Thomson; Edmund B. Rucker III; Jorge A. Piedrahita is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Mutational analysis of loxP sites for efficient Cre-mediated insertion into genomic DNA about?

## Abstract Summary: The Cre/__loxP__ system has been used in transgenic models primarily to excise DNA flanked by __loxP__ sites for gene deletion. However, the insertion reaction is more difficult to control since the excision event is kinetically favored. Mutant __loxP__ sites favoring integration were identified using a novel, bacterial screening system. Utilizing λ integrase, mutant __loxP__ sites were placed at the __E. coli attB__ site and the excision‐insertion ratios of incoming DNA plasmids carrying a second, complementary mutant __loxP__ site were determined. Comparison of 50 mutant __loxP__ sites combinations to the native __loxP__ site revealed that mutations to the inner 6 bp of the Cre binding domain severely inhibited recombination, while those in the outer 8 bps were more tolerated. The most efficient __loxP__ combinations resulted in 1,421‐fold and 1,529‐fold increases in relative integration rates over wild‐type __loxP__ sites. These __loxP__ mutants could be exploited for site‐directed “tag and insert” recombination experiments. genesis 36:162–167, 2003. © 2003 Wiley‐Liss, Inc.

Who reads Mutational analysis of loxP sites for efficient Cre-mediated insertion into genomic DNA?

Author

James G. Thomson; Edmund B. Rucker III; Jorge A. Piedrahita

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1526-954X)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)