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Rapid Modeling of Two-dimensional Multi-photon Excitation Microscopic Imaging Through Turbid Medium by Qiang Lu; Shaoqun Zeng; Qingming Luo; Yu Ruan is a Engineering article available to read on EtoBox.

What is Rapid Modeling of Two-dimensional Multi-photon Excitation Microscopic Imaging Through Turbid Medium about?

A rapid model to study two-dimensional (2-D) multi-photon excitation (MPE) ̄uorescence microscopic imaging through turbid medium has been presented. This model combines traditional Monte Carlo method with 2-D point spread function (PSF). Planar ̄uorescent object was used to calculate the 2-D PSF, which avoided the simulation of point scanning in the direct Monte Carlo method. In the meantime, this rapid model has generalized program logic and need only one-step Monte Carlo calculation for the simulations of single, two-and three-photon excitation ̄uorescence microscopic imaging. Experimental results showed that the rapid model using convolution operation was much faster than the direct Monte Carlo method. Similar computation eciency was achieved for dierent order of MPE. This model provides high potential in the simulation of MPE ̄uorescence imaging.

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Author

Qiang Lu; Shaoqun Zeng; Qingming Luo; Yu Ruan

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0030-4018)

Published

2001

Language

EN

Field

Engineering (Physical Sciences)