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Activation of Protein Kinase C Triggers Irreversible Cell CycleWithdrawal In Human Keratinocytes by Tibudan, Shalini S.; Wang, Yihua; Denning, Mitchell F. is a Medicine article available to read on EtoBox.

What is Activation of Protein Kinase C Triggers Irreversible Cell CycleWithdrawal In Human Keratinocytes about?

Irreversible cell cycle withdrawal occurs as normal keratinocytes detach from the basement membrane and initiate their terminal differentiation program. To investigate which signaling pathways regulate this permanent cell cycle withdrawal, we added inhibitors of kinases implicated in integrin signaling and keratinocyte differentiation to normal human keratinocytes induced to differentiate in suspension culture, and assayed the growth capacity of the recovered cells. Keratinocytes suspended in methylcellulose for 24 h underwent approximately 1000-fold loss of proliferative capacity. Of the kinase inhibitors tested, only the protein kinase C inhibitor Bisindolylmaleimide I (GF109203X) caused dramatic protection from loss of growth potential. Direct activation of protein kinase C by 12-O-tetradecanoyl-phorbol-13-acetate was also sufficient to trigger irreversible growth arrest. Protein kinase C inhibitors selective for protein kinase Calpha, the only Ca2+-dependent protein kinase C isoform in keratinocytes, protected keratinocytes from suspension-induced cell cycle withdrawal. Consistent with this finding, we measured a specific induction of Ca2+-dependent protein kinase C activity 2-

Who reads Activation of Protein Kinase C Triggers Irreversible Cell CycleWithdrawal In Human Keratinocytes?

Author

Tibudan, Shalini S.; Wang, Yihua; Denning, Mitchell F.

Publisher

Nature Publishing Group; Elsevier ; Elsevier Inc.; Elsevier BV; Springer Science and Business Media LLC; Wiley (ISSN 0022-202X)

Published

2002

Language

EN

Field

Medicine (Health Sciences)