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Crystal structure of the 2[4Fe-4S] ferredoxin from Chromatium vinosum: Evolutionary and mechanistic inferences for [3/4Fe-4S] ferredoxins by Jean-Marc Moulis; Larry C. Sieker; Keith S. Wilson; Zbigniew Dauter is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The crystal structure of the 2[4Fe‐4S] ferredoxin from __Chromatium vinosum__ has been solved by molecular replacement using data recorded with synchrotron radiation. The crystals were hexagonal prisms that showed a strong tendency to develop into long tubes. The hexagonal prisms diffracted to 2.1 Å resolution at best, and a structural model for C. __vinosum__ ferredoxin has been built with a final __R__ of 19.2%. The N‐terminal domain coordinates the two [4Fe‐4S] clusters in a fold that is almost identical to that of other known ferredoxins. However, the structure has two unique features. One is a six‐residue insertion between two ligands of one cluster forming a two‐turn external loop; this short loop changes the conformation of the Cys 40 ligand compared to other ferredoxins and hampers the building of one NH—S H‐bond to one of the inorganic sulfurs. The other remarkable structural element is a 3.5‐turn α‐helix at the C‐terminus that covers one side of the same cluster and is linked to the cluster‐binding domain by a six‐residue external chain segment. The charge distribution is highly asymmetric over the molecule. The structure of __C. vinosum__ ferredoxin strongly

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Author

Jean-Marc Moulis; Larry C. Sieker; Keith S. Wilson; Zbigniew Dauter

Publisher

Cold Spring Harbor Laboratory Press; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 0961-8368)

Published

1996

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)