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Effect of human patient plasma ex vivo treatment on gene expression and progenitor cell activation of primary human liver cells in multi-compartment 3D perfusion bioreactors for extra-corporeal liver support by Eva Schmelzer; Kerim Mutig; Petra Schrade; Sebastian Bachmann; Jörg C Gerlach; Katrin Zeilinger is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Cultivation of primary human liver cells in innovative 3D perfusion multi‐compartment capillary membrane bioreactors using decentralized mass exchange and integral oxygenation provides in vitro conditions close to the physiologic environment in vivo. While a few scale‐up bioreactors were used clinically, inoculated liver progenitors in these bioreactors were not investigated. Therefore, we characterized regenerative processes and expression patterns of auto‐ and paracrine mediators involved in liver regeneration in bioreactors after patient treatment. Primary human liver cells containing parenchymal and non‐parenchymal cells co‐cultivated in bioreactors were used for clinical extra‐corporeal liver support to bridge to liver transplantation. 3D tissue re‐structuring in bioreactors was studied; expression of proteins and genes related to regenerative processes and hepatic progenitors was analyzed. Formation of multiple bile ductular networks and colonies of putative progenitors were observed within parenchymal cell aggregates. HGF was detected in scattered cells located close to vascular‐like structures, expression of HGFA and c‐Met was assigned to biliary cells and hepat

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Author

Eva Schmelzer; Kerim Mutig; Petra Schrade; Sebastian Bachmann; Jörg C Gerlach; Katrin Zeilinger

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)