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Enhanced CO2 capture in packed-bed column bioreactors with immobilized carbonic anhydrase by Hannaneh Rasouli; Ion Iliuta; Francis Bougie; Alain Garnier; Maria C. Iliuta is a Engineering article available to read on EtoBox.

What is Enhanced CO2 capture in packed-bed column bioreactors with immobilized carbonic anhydrase about?

A novel hybrid enzymatic process with human Carbonic Anhydrase II (hCA II) enzyme immobilized on packing surface and magnetic nanoparticles (MNPs) dispersed in liquid absorbent in a gas-liquid packed-bed column bioreactor was proposed. Packing and MNPs surface were amine-functionalized by co-deposition of polydopamine (PDA)/polyethyleneimine (PEI) and hCA II was immobilized on the modified surface via glutaraldehyde. Immobilized hCA II on packing and MNPs displayed a high storage stability even after 40 days, preserving 80% and 84.7% of their initial activities, respectively. CO 2 hydration process achieved significant improvements due to the additional hCA II enzyme immobilized on the MNPs surface, functioning as a free solution-phase enzyme, especially when the diffusion limitation of the enzymatic process with immobilized hCA II enzyme on the packing surface was very significant. CO 2 absorption performance has been evaluated experimentally and theoretically under different conditions, including buffer type, buffer concentration, and operational parameters. A 3-D model describing the hydrodynamics, mass transport/reaction in liquid/gas phases, accumulation of biocatalytic MNPs i

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Author

Hannaneh Rasouli; Ion Iliuta; Francis Bougie; Alain Garnier; Maria C. Iliuta

Publisher

Elsevier BV

Published

2022

Language

EN

Field

Engineering (Physical Sciences)