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Stimulation of Human Amniotic Fluid Cell Proliferation and Colony Formation by Cell Plating on a Naturally Produced Extracellular Matrix by I. Vlodavsky; R. Voss; S. Yarkoni; Z. Fuks is a Medicine article available to read on EtoBox.

What is Stimulation of Human Amniotic Fluid Cell Proliferation and Colony Formation by Cell Plating on a Naturally Produced Extracellular Matrix about?

## Abstract Human amniotic fluid cells exhibit a higher cloning efficiency and rate of cell proliferation when maintained on dishes coated with a naturally produced extracellular matrix (ECM) in comparison with the regular tissue culture plastic. In 22 out of 31 amniotic fluid samples there was by plating the cells on ECM a 2–6 fold increase in number and size of colonies and in the cell density per colony as detected by actual staining and viewing of each colony. These effects yielded, in 21 of 41 additional samples, a reduction ranging from 2–8 days, in the culture time elapsing between amniocentesis and the first harvesting of cells for chromosomal analysis. An even greater effect was obtained with primary cells that failed to attach to plastic surfaces and stayed floating in the medium but did attach and proliferate when seeded on ECM. Cells that were left firmly attached to ECM after the first trypsinization and harvesting of cells for chromosomal analysis yielded colonies ready for second karyotyping in less than half the time required for cells maintained on plastic. Studies with secondary cultures of human amniotic fluid cells have demonstrated a 5–10 fold decrease in serum

Who reads Stimulation of Human Amniotic Fluid Cell Proliferation and Colony Formation by Cell Plating on a Naturally Produced Extracellular Matrix?

Author

I. Vlodavsky; R. Voss; S. Yarkoni; Z. Fuks

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0197-3851)

Published

1982

Language

EN

Field

Medicine (Health Sciences)