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Evaluation of an Integrated Strategy for Proteomic Profiling of Skeletal Muscle by Marie‐Catherine Le Bihan; Edward Tarelli; Gary R. Coulton is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Proteomic analysis of skeletal muscle presents particular challenges when trying to identify valid biomarkers of phenotypic change in small biopsies from genetically diverse human subjects. Currently, two‐dimensional (2‐D) gel electrophoresis and mass spectrometry are the chosen analytical strategies but 2‐D gels are not appropriate for analyzing proteins less than 11 kDa, they can suffer from problems of reproducibility and in routine use are not a viable high‐throughput technique. We have evaluated an integrated proteomic strategy employing Ciphergen ProteinChip® arrays, one‐dimensional polyacrylamide gel electrophoresis and mass spectrometry. Protein fingerprints characteristic of fast and slow contracting muscles from normal and kyphoscoliosis (ky) mutant mice were obtained from Ciphergen protein arrays. Eight statistically validated protein biomarkers have so far been identified capable of discriminating fast from slow muscle. Five of these showed further differential expression in ky __versus__ normal BDL soleus muscles. Several biomarkers have been formally identified, and were myosin light chain isoforms shown previously to be expressed differentially by fast __

Who reads Evaluation of an Integrated Strategy for Proteomic Profiling of Skeletal Muscle?

Author

Marie‐Catherine Le Bihan; Edward Tarelli; Gary R. Coulton

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)

Published

2004

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)