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Effects of the marine toxin palytoxin on human skin keratinocytes: Role of ionic imbalance by M. Pelin; C. Zanette; M. De Bortoli; S. Sosa; R. Della Loggia; A. Tubaro; C. Florio is a Environmental Science article available to read on EtoBox.

What is Effects of the marine toxin palytoxin on human skin keratinocytes: Role of ionic imbalance about?

Palytoxin (PLTX), a marine toxin identified in Palythoa zoanthid corals and Ostreopsis dinoflagellates, represents an increasing hazard for human health. Recently, dermatological problems have been associated to cutaneous exposure to PLTX during Ostreopsis blooms arising the need for experimental data characterizing PLTX effects on the skin. This study highlights in vitro the cytotoxic effects of PLTX on human keratinocytes (HaCaT cell line). A short time exposure (4 h) to PLTX reduced mitochondrial activity (MTT assay), cell mass (SRB assay) and plasma membrane integrity (LDH leakage) with different potencies: EC 50 values of 6.1 ± 1.3 × 10 -11 , 4.7 ± 0.9 × 10 -10 M and 1.8 ± 0.1 × 10 -8 M, respectively. PLTX effect on mitochondrial activity was ouabain-and Na + -sensitive, but only partially sensitive to removal of Ca 2+ ions. One hour exposure to the toxin also induced a Na + -dependent and Ca 2+ -independent superoxide anion production. These results indicate that among the chain of intracellular events following the interaction of PLTX with the Na + /K + -ATPase the first and crucial step is the increased intracellular Na + concentration that triggers a sequence of cell dysfu

Who reads Effects of the marine toxin palytoxin on human skin keratinocytes: Role of ionic imbalance?

Author

M. Pelin; C. Zanette; M. De Bortoli; S. Sosa; R. Della Loggia; A. Tubaro; C. Florio

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0300-483X)

Published

2011

Language

EN

Field

Environmental Science (Life Sciences)