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Transgenesis Techniques Volume 180 by Clarke, Alan R. is a scholarly article available to read on EtoBox.

What is Transgenesis Techniques Volume 180 about?

The past decade has witnessed a spectacular explosion in both the development and the use of transgenic technologies. In Transgenesis Techniques: Principles and Protocols, Second Edition, authoritative researchers-including members of the Dolly team-take stock of these exciting developments to present a collection of readily reproducible techniques for the generation and analysis of transgenic animals, focusing on the manipulation of the mammalian genome. This second edition devotes much space to the creation of genetically modified murine strains, providing access to the germline both by conventional pronuclear injection and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms. Comprehensive and highly practical, this second edition of Transgenesis Techniques: P

Author

Clarke, Alan R.

Publisher

Humana Press

Published

2002

Language

EN

ISBN

9781489939012