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Can I read Radioimmunotherapy of Fibroblast Activation Protein Positive Tumors by Rapidly Internalizing Antibodies on EtoBox?
Radioimmunotherapy of Fibroblast Activation Protein Positive Tumors by Rapidly Internalizing Antibodies by Eliane Fischer; Krishna Chaitanya; Thomas Wüest; Andreas Wadle; Andrew M. Scott; Maries van den Broek; Roger Schibli; Stefan Bauer; Christoph Renner is a Medicine article available to read on EtoBox.
What is Radioimmunotherapy of Fibroblast Activation Protein Positive Tumors by Rapidly Internalizing Antibodies about?
## Abstract Purpose: Fibroblast activation protein (FAP) is a serine protease that has emerged as a promising target for cancer therapy, either by direct abrogation of its proinvasive activity or by specific targeting of FAP-expressing cells with cytotoxic immunoconjugates. We aimed to select novel human–mouse cross-reactive antibodies and to test suitability for tumor therapy as radioimmunoconjugates in a preclinical model. Experimental Design: Human Fab fragments that bind to human and murine FAP were selected from an antibody phage library. Two candidates (ESC11 and ESC14) were engineered into fully human IgG1 antibodies and further characterized. We investigated the intracellular trafficking of ESC11 and ESC14 in live cells by confocal microscopy and analyzed the biodistribution and therapeutic effects of anti-FAP antibodies labeled with the β-emitting radionuclide 177Lu in a melanoma xenograft nude mouse model. Results were compared with vF19, a humanized variant of an anti-FAP antibody that has been previously used in clinical trials. Results: The two antibodies bound selectively to both human and mouse FAP, with affinities in the low nanomolar range. Binding to FAP-expressin
Who reads Radioimmunotherapy of Fibroblast Activation Protein Positive Tumors by Rapidly Internalizing Antibodies?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Eliane Fischer; Krishna Chaitanya; Thomas Wüest; Andreas Wadle; Andrew M. Scott; Maries van den Broek; Roger Schibli; Stefan Bauer; Christoph Renner
- Publisher
- American Association for Cancer Research (AACR)
- Published
- 2012
- Language
- EN
- Field
- Medicine (Health Sciences)