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Can I read Crystallization and preliminary X-ray diffraction studies of β-phosphoglucomutase from Lactococcus lactus on EtoBox?
Crystallization and preliminary X-ray diffraction studies of β-phosphoglucomutase from Lactococcus lactus by Sushmita D. Lahiri; Guofeng Zhang; Peter Radstrom; Debra Dunaway-Mariano; Karen N. Allen is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Crystallization and preliminary X-ray diffraction studies of β-phosphoglucomutase from Lactococcus lactus about?
Phosphoglucomutase (-PGM), a 28 kDa monomer, catalyzes the reversible conversion of -d-glucose-1-phosphate to -d-glucose-6-phosphate in maltose metabolism in a variety of organisms. Sequence analysis of -PGM indicates that it is a member of the haloacid dehalogenase (HAD) enzyme superfamily, which evolved to cleave CÐCl, CÐP and CÐOP bonds in a variety of substrates. -PGM has been crystallized using the hanging-drop method. Diffraction-quality crystals of the native protein have been obtained from two conditions, both belonging to space group P2 1 2 1 2 1 , with unit-cell parameters a = 53.67, b = 92.78, c = 111.60 and a = 53.21, b = 57.01, c = 76.11 A Ê . To solve the phase problem, selenomethionine (SeMet) containing -PGM crystals have been grown. The SeMetcontaining crystals diffract to high resolution only when grown by microseeding with native crystals. A three-wavelength data set has been collected to 2.3 A Ê on crystals of the SeMet-substituted -PGM. The structure solution is currently being attempted by the multiwavelength anomalous diffraction (MAD) phasing method.
Who reads Crystallization and preliminary X-ray diffraction studies of β-phosphoglucomutase from Lactococcus lactus?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Sushmita D. Lahiri; Guofeng Zhang; Peter Radstrom; Debra Dunaway-Mariano; Karen N. Allen
- Publisher
- International Union of Crystallography; Blackwell Publishing Inc.; International Union of Crystallography (IUCr) (ISSN 0907-4449)
- Published
- 2002
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)