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Development of an in vitro model of tumor progression using v‐ raf and v‐ raf /v‐ myc transformed rat liver epithelial cells: Correlation of tumorigenicity with the downregulation of specific proteins by Peter J. Worland; Lori L. Hampton; Snorri S. Thorgeirsson; Anthony C. Huggett is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract A series of clonal cell lines were derived from rat liver epithelial cells after being infected with a defective retrovirus containing either v‐__raf__ (3611‐MSV) or v‐__raf__/v‐__myc__(J2) together with a helper virus. These clones exhibited a different morphology from the regular cuboid shape of the control cells, infected only with the helper virus. All of the infected cell lines contained at least one full length copy of appropriate proviral DNA and expressed comparable levels of v‐__raf__ mRNA, although only the cells transformed with the v‐__raf__/v‐__myc__ combination were capable of anchorage‐independent growth in soft agar. All of the clones except the controls formed tumors in nude mice but with markedly different latency periods and growth rates. Thus, these cell lines represent an in vitro model for tumor progression. Two‐dimensional polyacrylamide electrophoresis was used to investigate changes in cellular protein expression related to malignant conversion. The expression of three proteins of pl/M~r~ × 10^−3^ 5.9‐7.2/205 (RP1), 6.5‐7.5/160 (RP2) and 4.0/85 (RP3) consistently matched the transformed phenotype. In particular the expression of RP1 and RP2 corr

Who reads Development of an in vitro model of tumor progression using v‐ raf and v‐ raf /v‐ myc transformed rat liver epithelial cells: Correlation of tumorigenicity with the downregulation of specific proteins?

Author

Peter J. Worland; Lori L. Hampton; Snorri S. Thorgeirsson; Anthony C. Huggett

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0899-1987)

Published

1990

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)