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Identification and quantitative analysis of cytokinins from shoot apices of Mercurialis ambigua by gas chromatography?Mass spectrometry computer system by B. Dauphin; G. Teller; B. Durand is a Agricultural and Biological Sciences article available to read on EtoBox.

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This paper describes the identification and quantitative analysis of cytokinins from natural sources (150-500 g fresh weight) at the submicrogram level. It summarizes an improved purification procedure with high resolution power that permits the detection of Trimethylsilylderivatives by gas chromatography-mass spectrometry. A comparison of the intensity of a characteristic ion in the mass spectrum of suitable standard(5 gg)and theintensity of the same ion in the mass spectrum of the extraction product permitted precise quantitative analysis. The method has been used to determine zeatin, trans-and cis-ribosylzeatin, and A2-isopentenyladenosine concentrations in extracts from female and monoecious MercuriaIis ambigua apices. It has been proved that differences appear in the endogenous cytokinin pools of monoecious and female individuals.

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Author

B. Dauphin; G. Teller; B. Durand

Publisher

Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0032-0935)

Published

1979

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)