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Metformin alleviates the calcification of aortic valve interstitial cells through activating the PI3K/AKT pathway in an AMPK dependent way by Qiao En; Huang Zeping; Wang Yuetang; Wang Xu; Wang Wei is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Metformin alleviates the calcification of aortic valve interstitial cells through activating the PI3K/AKT pathway in an AMPK dependent way about?

## Background: Calcific aortic valve disease (cavd) is the most prevalent valvular disease worldwide. however, no effective treatment could delay or prevent the progression of the disease due to the poor understanding of its pathological mechanism. many studies showed that metformin exerted beneficial effects on multiple cardiovascular diseases by mediating multiple proteins such as ampk, nf-κb, and akt. this study aims to verify whether metformin can inhibit aortic calcification through the pi3k/akt signaling pathway. ## Methods: We first analyzed four microarray datasets to screen differentially expressed genes (degs) and signaling pathways related to cavd. then aortic valve samples were used to verify selected genes and pathways through immunohistochemistry (ihc) and western blot (wb) assays. aortic valve interstitial cells (avics) were isolated from non-calcific aortic valves and then cultured with phosphate medium (pm) with or without metformin to verify whether metformin can inhibit the osteogenic differentiation and calcification of avics. finally, we used inhibitors and sirna targeting ampk, nf-κb, and akt to study the mechanism of metformin. ## Results: We screened 227 deg

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Author

Qiao En; Huang Zeping; Wang Yuetang; Wang Xu; Wang Wei

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)