Opening book details…
Can I read Improved ballistic transient transformation conditions for tomato fruit allow identification of organ-specific contributions of I-box and G-box to the RBCS2 promoter activity on EtoBox?
Improved ballistic transient transformation conditions for tomato fruit allow identification of organ-specific contributions of I-box and G-box to the RBCS2 promoter activity by Katja Baum; Birge Gröning; Iris Meier is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Improved ballistic transient transformation conditions for tomato fruit allow identification of organ-specific contributions of I-box and G-box to the RBCS2 promoter activity about?
An improved protocol for the ballistic transient transformation of developing tomato (__Lycopersicon esculentum__) fruits is reported, which allows high‐resolution __cis__‐analysis of fruit‐specific transcriptional activation. The tomato __RBCS2__ promoter fused to the firefly luciferase gene was used as a model system for this study. Osmotic treatment of fruit slices before, during and after particle bombardment, together with the optimization of bombardment conditions, resulted in a 100‐fold increase in __RBCS2__ promoter‐driven transient luciferase expression compared with previously reported protocols. Under these conditions, the transformed __RBCS2__ promoter was shown to be properly regulated in a developmental fashion. A __cis__‐analysis of the __RBCS2__ promoter was performed. A 37 bp domain is required for high‐level __RBCS2__ promoter activity both in leaves and young fruits. Two conserved sequence elements within this domain, an I‐box element (GATAAG) and a G‐box element (CACGTG), are necessary for its activity in leaves. In contrast, in young fruits, the G‐box is the single dominant __cis__‐acting element. These findings are discussed with respect to the proposed functi
Who reads Improved ballistic transient transformation conditions for tomato fruit allow identification of organ-specific contributions of I-box and G-box to the RBCS2 promoter activity?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Katja Baum; Birge Gröning; Iris Meier
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7412)
- Published
- 1997
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)