Can I read Ultrahigh Molecular Weight Polyethylene Wear Debris Inhibits Osteoprogenitor Proliferation and Differentiation in Vitro on EtoBox?

Ultrahigh Molecular Weight Polyethylene Wear Debris Inhibits Osteoprogenitor Proliferation and Differentiation in Vitro by Richard Chiu; Ting Ma; R. Lane Smith; Stuart B. Goodman is a Engineering article available to read on EtoBox.

What is Ultrahigh Molecular Weight Polyethylene Wear Debris Inhibits Osteoprogenitor Proliferation and Differentiation in Vitro about?

## Abstract Polyethylene wear debris induces progressive osteolysis by increasing bone degradation and suppressing bone formation. Polyethylene particles inhibit the function of mature osteoblasts, but whether polyethylene particles also interfere with the proliferation and differentiation of osteoprogenitor cells is unknown. In this study, we investigated the effects of ultrahigh molecular weight polyethylene (UHMWPE) particles on the osteogenic activity of primary murine bone marrow osteoprogenitors and MC3T3‐E1 preosteoblastic cells __in vitro__. Submicron‐sized UHMWPE particles generated from wear simulator tests were isolated from serum‐containing solution by density gradient centrifugation. The particles were coated onto the surface of culture wells at concentrations of 0.038, 0.075, 0.150, 0.300, and 0.600% v/v in a layer of type I collagen matrix. Primary murine bone marrow cells and MC3T3‐E1 preosteoblasts were seeded onto the particle‐collagen matrix and induced to differentiate in osteogenic medium for 20 days. Exposure of both cell populations to UHMWPE particles resulted in a dose‐dependent decrease in mineralization, proliferation, alkaline phosphatase activity, and o

Who reads Ultrahigh Molecular Weight Polyethylene Wear Debris Inhibits Osteoprogenitor Proliferation and Differentiation in Vitro?

Author

Richard Chiu; Ting Ma; R. Lane Smith; Stuart B. Goodman

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1549-3296)

Published

2008

Language

EN

Field

Engineering (Physical Sciences)