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Molecular recognition between oligopeptides and nucleic acids. DNA sequence specificity and binding properties of an acridine-linked netropsin hybrid ligand by Christian Bailly; Nicole Helbecque; Jean-Pierre Hénichart; Pierre Colson; Claude Houssier; K. Ekambareswara Rao; Regan G. Shea; J. William Lown is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The binding to DNA of a mixed function ligand (NETGA) is described, in which a potential intercalating group, an acridine moiety, is incorporated at the carboxyl terminus of the minor groove binding oligopeptide netropsin skeleton. Scatchard analysis of absorption data provided evidence of two modes of binding to DNA with __K__~1~ = 9.1 × 10^5^ M^−1^ at low r values (0.003–0.1), and a binding site size __n__ = 10, indicative of binding of both moeities. At high binding ratios (>0.1), __K__~2~ = 0.9 × 10^5^ M^−1^ and __n__ = 5 corresponding to external binding. Complementary strand MPE footprinting on a pBR322 restriction fragment showed NETGA binds to 5′‐AAAT like netropsin. It causes enhanced cleavage by MPE, particularly at G‐C rich sequences and remote from the preferred binding sites. Viscometry measurements provided evidence for biphasic modes of the two binding portions of NETGA. Fluorescence polarization and linear dichroism measurements were in accord with distinct modes of interaction of the acridine (intercalation) and oligopeptide (minor groove binding) portions of NETGA. LD measurments of NETGA indicate that the oligopeptide moiety (netropsin‐like) has an or

Who reads Molecular recognition between oligopeptides and nucleic acids. DNA sequence specificity and binding properties of an acridine-linked netropsin hybrid ligand?

Author

Christian Bailly; Nicole Helbecque; Jean-Pierre Hénichart; Pierre Colson; Claude Houssier; K. Ekambareswara Rao; Regan G. Shea; J. William Lown

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0952-3499)

Published

1990

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)