About this document
Bradford Assay Protocol for Protein Estimation by KL Tongson is a document available to read on EtoBox.
The document describes the Bradford assay, a simple and accurate method for determining total protein concentration. It binds the dye Coomassie Brilliant Blue G-250 to proteins, causing a color change. A standard curve using a protein like BSA or IgG is made to determine concentrations of unknown samples. The assay is fast, sensitive, and not susceptible to many chemicals, though detergents can interfere. Sample preparation and the measurement of absorbance are explained.
- Author
- KL Tongson
- Language
- EN