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Reversibility of Metabolic and Morphological Changes Associated with Chronic Exposure of Pancreatic Islet β-Cells to Fatty Acids by Katherine Pinnick; Matt Neville; Anne Clark; Barbara Fielding is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Pancreatic β‐cells metabolise both lipid and glucose nutrients but chronic exposure (>24 h) to elevated fatty acid (FA) concentrations results in deleterious metabolic and morphological changes. The aims of this study were to assess the adaptive morphological, metabolic and secretory responses of islet β‐cells to exposure and removal of FA. Isolated mouse islets and INS‐1 β‐cells were exposed to oleate or palmitate (0.5 mM) or a 1:1 mixture of both FA for 48 h prior to a 24 h period without FA. Subsequent changes in lipid storage and composition (triglycerides, TG and phospholipids, PL), gene expression, β‐cell morphology and glucose‐stimulated insulin secretion (GSIS) were determined. Intracellular TG content increased during exposure to FA and was lower in cells subsequently incubated in FA‐free media (__P__ < 0.05); TG storage was visible as oil red O positive droplets (oleate) by light microscopy or ‘splits’ (palmitate) by electron microscopy. Significant desaturation of β‐cell FA occurred after exposure to oleate and palmitate. After incubation in FA‐free media, there was differential handling of specific FA in TG, resulting in a profile that tended to revert to th

Who reads Reversibility of Metabolic and Morphological Changes Associated with Chronic Exposure of Pancreatic Islet β-Cells to Fatty Acids?

Author

Katherine Pinnick; Matt Neville; Anne Clark; Barbara Fielding

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

2010

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)