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In vitro differentiation of natural killer T cells from human cord blood CD34^+^ cells by So‐Youn Woo; Yu‐Jin Jung; Kyung‐Ha Ryu; Hae‐Young Park; Jeong‐Hae Kie; Seok‐Ah Im; Wha‐Soon Chung; Ho‐Seong Han; Ju‐Young Seoh is a Medicine article available to read on EtoBox.

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**Summary.** Natural killer T (NKT) cells are involved in innate immune defence and also in the regulation of adaptive immune responses. However, the development of NKT cells __in vitro__ has not been fully characterized and culture conditions have not been fully optimized. In the present study, we found that an NKT cell fraction developed during the __in vitro__ culture of cord blood (CB) CD34^+^ cells, and this was subsequently characterized both phenotypically and morphologically. CD34^+^ cells purified from 10 human CB were cultured in the presence of several cytokines and analysed by flow cytometry, light microscopy and electron microscopy. The NKT cell fraction, defined phenotypically (CD3^+^CD16^+^CD56^+^CD94^+^) as expressing the invariant T‐cell receptor Vα24 and Vβ11, appeared in the CD56^hi^ fractions. Intracytoplasmic staining demonstrated that interferon‐γ and interleukin 4 (IL‐4) were detected in the CD56^hi^ fractions. IL‐15 was essential and, in combination with either flt3‐ligand (FL) or stem cell factor (SCF), was sufficient to induce the development of NKT cells. The phenotype of the NKT cell fraction was CD45RO^+^CD45RA^–^ and CD4^+^CD8α^+^. Morphologically, the

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Author

So‐Youn Woo; Yu‐Jin Jung; Kyung‐Ha Ryu; Hae‐Young Park; Jeong‐Hae Kie; Seok‐Ah Im; Wha‐Soon Chung; Ho‐Seong Han; Ju‐Young Seoh

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0007-1048)

Published

2003

Language

EN

Field

Medicine (Health Sciences)