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Guinea Pig Histamine H~1~ Receptor. I. Gene Cloning, Characterization, and Tissue Expression Revealed by In Situ Hybridization by E. Traiffort; R. Leurs; J. M. Arrang; J. Tardivel-Lacombe; J. Diaz; J.-C. Schwartz; M. Ruat is a Neuroscience article available to read on EtoBox.
What is Guinea Pig Histamine H~1~ Receptor. I. Gene Cloning, Characterization, and Tissue Expression Revealed by In Situ Hybridization about?
Abstract: An intronless DNA encoding the guinea pig H~1~ receptor was cloned from a genomic library using probes derived from the bovine H~1~ receptor. It encodes a protein of 488 amino acids with a calculated molecular mass of 55,619 daltons compared with a size of 56–68 kDa for the photoaffinity‐labeled receptor as determined by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis analysis. The protein displays a 66% homology with the bovine receptor. Stable expression of the H~1~ receptor, characterized by the appearance of [^3^H]mepyramine binding sites with a pharmacology similar to that of the native H~1~ receptor, was obtained following transfection of Chinese hamster ovary cells. Southern blot analysis, using a variety of restriction enzymes, did not provide any evidence of multiple H~1~ isoreceptors. Northern blot analysis of a variety of guinea pig peripheral or cerebral tissues identified, in most cases, a single transcript of 3.3 kb, but also, in some tissues, a second transcript of 3.7 kb, possibly generated by the use of different promoter or polyadenylation sites or corresponding to a transcript from a distinct gene. In situ hybridization studies showed the high
Who reads Guinea Pig Histamine H~1~ Receptor. I. Gene Cloning, Characterization, and Tissue Expression Revealed by In Situ Hybridization?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- E. Traiffort; R. Leurs; J. M. Arrang; J. Tardivel-Lacombe; J. Diaz; J.-C. Schwartz; M. Ruat
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)
- Published
- 1994
- Language
- EN
- Field
- Neuroscience (Life Sciences)