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Radiosensitization by 2-Benzoyl-3-phenyl-6,7-dichloroquinoxaline 1,4-Dioxide Under Oxia and Hypoxia in Human Colon Cancer Cells by Wafica Itani; Fady Geara; Joelle Haykal; Makhluf Haddadin; Hala Gali-Muhtasib is a Medicine article available to read on EtoBox.

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## Background The sensitizing effects of 2-benzoyl-3-phenyl-6,7-dichloroquinoxaline 1,4-dioxide (DCQ) and ionizing radiation (IR) were determined in four colon cancer cells and in FHs74Int normal intestinal cells. ## Methods Cell cycle modulation, TUNEL assay, clonogenic survival and DNA damage were examined under oxia or hypoxia. Effects on apoptotic molecules and on p-Akt and Cox-2 protein expression were investigated. ## Results The four cell lines responded differently to DCQ+IR; HT-29 cells were most resistant. Combination treatment caused significant increases in preG~1~ (apoptosis) in HCT-116, while G~2~/M arrest occurred in DLD-1. DCQ potentiated IR effects more so under hypoxia than oxia. Pre-exposure of DLD-1 to hypoxia induced 30% apoptosis, and G~2~/M arrest in oxia. The survival rate was 50% lower in DCQ+IR than DCQ alone and this rate further decreased under hypoxia. FHs74Int normal intestinal cells were more resistant to DCQ+IR than cancer cells.Greater ssDNA damage occurred in DLD-1 exposed to DCQ+IR under hypoxia than oxia. In oxia, p-Akt protein expression increased upon IR exposure and drug pre-treatment inhibited this increase. In contrast, in hypoxia, exposure

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Author

Wafica Itani; Fady Geara; Joelle Haykal; Makhluf Haddadin; Hala Gali-Muhtasib

Publisher

BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central, 2006-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1748-717X)

Published

2007

Language

EN

Field

Medicine (Health Sciences)