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Label-free detection of 16S ribosomal RNA hybridization on reusable DNA arrays using surface plasmon resonance imaging by Bryce P. Nelson; Mark R. Liles; Kendra B. Frederick; Robert M. Corn; Robert M. Goodman is a Environmental Science article available to read on EtoBox.

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## Summary In this paper, we describe the detection of bacterial cell‐extracted 16S ribosomal RNA (rRNA) using an emerging technology, surface plasmon resonance (SPR) imaging of DNA arrays. Surface plasmon resonance enables detection of molecular interactions on surfaces in response to changes in the index of refraction, therefore eliminating the need for a fluorescent or radioactive label. A variation of the more common SPR techniques, SPR imaging enables detection from multiple probes in a reusable array format. The arrays developed here contain DNA probes (15–21 bases) designed to be complementary to 16S rRNA gene sequences of __Escherichia coli__ and __Bacillus subtilis__ as well as to a highly conserved sequence found in rRNAs from most members of the domain Bacteria. We report species‐specific hybridization of cell‐extracted total RNA and __in vitro__ transcribed 16S rRNA to oligonucleotide probes on SPR arrays. We tested multiple probe sequences for each species, and found that success or failure of hybridization was dependent upon probe position in the 16S rRNA molecule. It was also determined that one of the probes intended to bind 16S rRNA also bound an unknown protein. T

Who reads Label-free detection of 16S ribosomal RNA hybridization on reusable DNA arrays using surface plasmon resonance imaging?

Author

Bryce P. Nelson; Mark R. Liles; Kendra B. Frederick; Robert M. Corn; Robert M. Goodman

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 1462-2912)

Published

2002

Language

EN

Field

Environmental Science (Physical Sciences)