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Qualitative and quantitative determination of the major coumarins in Zushima by high performance liquid chromatography with diode array detector and mass spectrometry by Juan Su; Chuan Zhang; Wei Zhang; Yun-heng Shen; Hui-liang Li; Run-hui Liu; Xi Zhang; Xiao-Jia Hu; Wei-dong Zhang is a Chemistry article available to read on EtoBox.
What is Qualitative and quantitative determination of the major coumarins in Zushima by high performance liquid chromatography with diode array detector and mass spectrometry about?
A method, high-performance liquid chromatography coupled with diode array and electrospray tandem mass spectrometry (HPLC-DAD-ESI-MS), was developed to qualitatively identify and quantitatively determine the 10 major active coumarins of Zushima. The analysis was performed by using a ZORBAX SB-C18 analytical column (250 mm x 4.6 mm ID, 5 microm) at gradient elution of 0.5% aqueous formic acid and acetonitrile with diode array detection (325 nm). The method was validated for linearity, precision, accuracy, limit of detection and quantification. The proposed method was successfully applied for the qualitative and quantitative analysis of 10 coumarins in five different species of Zushima which had great variation on the contents of investigated coumarins.
Who reads Qualitative and quantitative determination of the major coumarins in Zushima by high performance liquid chromatography with diode array detector and mass spectrometry?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Juan Su; Chuan Zhang; Wei Zhang; Yun-heng Shen; Hui-liang Li; Run-hui Liu; Xi Zhang; Xiao-Jia Hu; Wei-dong Zhang
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 2009
- Language
- EN
- Field
- Chemistry (Physical Sciences)