Opening book details…
Can I read APE/Ref-1 responses to oxidative stress in aged rats on EtoBox?
APE/Ref-1 responses to oxidative stress in aged rats by Michael Edwards; David K. Rassin; Tadahide Izumi; Sankar Mitra; J. Regino Perez-Polo is a Neuroscience article available to read on EtoBox.
What is APE/Ref-1 responses to oxidative stress in aged rats about?
Chronic oxidative stress has been hypothesized to be a major contributor to the aging process. The continued exposure to reactive oxygen species (ROS) generated by oxidative metabolism or environmental sources can damage critical cellular structures and be responsible for some age-related pathology. The exposure of rodents to 100% oxygen, isobaric hyperoxia, increases ambient ROS levels and significantly increases apoptosis in brain. The deleterious effects of ROS also include increased lipid peroxidation, protein oxidation, and DNA damage. Although differences in the relative amounts of oxidative stress in young and old brains have been observed, the mechanisms responsible for impaired aging-associated DNA repair processes have not been characterized. We measured DNA levels of the DNA repair enzyme apurinic/ apyrimidinic endonuclease (APE/Ref-1) protein by Western blot analysis in the brains of young (3-month) and old (30-month) male rats exposed to isobaric hyperoxia. Given that APE/Ref-1 is the rate-limiting enzyme in the repair pathway of apurinic/apyrimidinic sites generated in DNA by oxidative damage, we assumed that APE/Ref-1 protein levels were a good reflection of ongoing
Who reads APE/Ref-1 responses to oxidative stress in aged rats?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- Michael Edwards; David K. Rassin; Tadahide Izumi; Sankar Mitra; J. Regino Perez-Polo
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0360-4012)
- Published
- 1998
- Language
- EN
- Field
- Neuroscience (Life Sciences)