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The Escherichia coli threonyl‐tRNA synthetase gene contains a split ribosomal binding site interrupted by a hairpin structure that is essential for autoregulation by Christine Sacerdot; Joel Caillet; Monique Graffe; Flore Eyermann; Bernard Ehresmann; Chantal Ehresmann; Mathias Springer; Pascale Romby is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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The expression of the gene encoding __Escherichia coli__ threonyl‐tRNA synthetase (ThrRS) is negatively autoregulated at the translational level. ThrRS binds to its own mRNA leader, which consists of four structural and functional domains: the Shine–Dalgarno (SD) sequence and the initiation codon region (domain 1); two upstream hairpins (domains 2 and 4) connected by a single‐stranded region (domain 3). Using a combination of __in vivo__ and __in vitro__ approaches, we show here that the ribosome binds to __thrS__ mRNA at two non‐contiguous sites: region −12 to +16 comprising the SD sequence and the AUG codon and, unexpectedly, an upstream single‐stranded sequence in domain 3. These two regions are brought into close proximity by a 38‐nucleotide‐long hairpin structure (domain 2). This domain, although adjacent to the 5′ edge of the SD sequence, does not inhibit ribosome binding as long as the single‐stranded region of domain 3 is present. A stretch of unpaired nucleotides in domain 3, but not a specific sequence, is required for efficient translation. As the repressor and the ribosome bind to interspersed domains, the competition between ThrRS and ribosome for __thrS__ mRNA binding

Who reads The Escherichia coli threonyl‐tRNA synthetase gene contains a split ribosomal binding site interrupted by a hairpin structure that is essential for autoregulation?

Author

Christine Sacerdot; Joel Caillet; Monique Graffe; Flore Eyermann; Bernard Ehresmann; Chantal Ehresmann; Mathias Springer; Pascale Romby

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)

Published

1998

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)