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Phosphosite Analysis of the Cytomegaloviral mRNA Export Factor pUL69 Reveals Serines with Critical Importance for Recruitment of Cellular Proteins Pin1 and UAP56/URH49 by Thomas, Marco; MÃ1⁄4ller, Regina; Horn, Georg; Bogdanow, Boris; Imami, Koshi; Milbradt, Jens; Steingruber, Mirjam; Marschall, Manfred; Schilling, Eva-Maria; Fossen, Torgils; Stamminger, Thomas; Sandri-G is a Medicine article available to read on EtoBox.

What is Phosphosite Analysis of the Cytomegaloviral mRNA Export Factor pUL69 Reveals Serines with Critical Importance for Recruitment of Cellular Proteins Pin1 and UAP56/URH49 about?

The multifunctional regulatory protein pUL69 of human cytomegalovirus acts as a viral RNA export factor with a critical role in efficient replication. Here, we identify serine/threonine phosphorylation sites for cellular and viral kinases within pUL69. We demonstrate that the pUL97/CDK phosphosites within alpha-helix 2 of pUL69 are crucial for its cis/trans isomerization by the cellular protein Pin1. Thus, we identified pUL69 as the first HCMV-encoded protein that is phosphorylated by cellular and viral serine/threonine kinases in order to serve as a substrate for Pin1. Furthermore, our study revealed that betaherpesviral mRNA export proteins contain extended binding motifs for the cellular mRNA adaptor proteins UAP56/URH49 harboring phosphorylated serines that are critical for efficient viral replication. Knowledge of the phosphorylation sites of pUL69 and the processes regulated by these posttranslational modifications is important in order to develop antiviral strategies based on a specific interference with pUL69 phosphorylation.

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Author

Thomas, Marco; MÃ1⁄4ller, Regina; Horn, Georg; Bogdanow, Boris; Imami, Koshi; Milbradt, Jens; Steingruber, Mirjam; Marschall, Manfred; Schilling, Eva-Maria; Fossen, Torgils; Stamminger, Thomas; Sandri-G

Publisher

American Society for Microbiology (ISSN 0022-538X)

Published

2020

Language

EN

Field

Medicine (Health Sciences)