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Can I read Mass spectrometric sequencing of endotoxin proteins of ** Bacillus thuringiensis ** ssp. ** konkukian ** extracted from polyacrylamide gels on EtoBox?
Mass spectrometric sequencing of endotoxin proteins of ** Bacillus thuringiensis ** ssp. ** konkukian ** extracted from polyacrylamide gels by Kwang Yong Lee; Eun Young Kang; Sooil Park; Seong Kyu Ahn; Kwan Hee Yoo; Jin Young Kim; Hyung Hoan Lee is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Mass spectrometric sequencing of endotoxin proteins of ** Bacillus thuringiensis ** ssp. ** konkukian ** extracted from polyacrylamide gels about?
## Abstract The amino acid sequences of the crystal proteins of __Bacillus thuringiensis__ ssp. __konkukian__ strain HL‐47 are unknown. We used 1‐D denaturing polyacrylamide electrophoresis, nano‐ESI‐Q‐TOF‐MS, and protein database searching to analyze these proteins. On SDS‐PAGE gels, a preparation of purified crystal proteins exhibited 110, 102, 76, 55, 37, and 30 kDa protein bands. Immunoblotting of the gel with antiserum raised to this preparation revealed that four crystal proteins, of 110, 102, 55, and 37 kDa, reacted with the specific antiserum. The 102‐kDa major protein reacted strongly. The other crystal proteins showed weak immunoreactivity. The 102 and 55 kDa proteins were analyzed by ESI‐MS. The internal amino acid sequence of the 102‐kDa major protein has similarity to the sequences of the surface layer protein of __B. thuringiensis__ ssp. __finitimus__ and __B. anthracis.__ However, the internal amino acid sequences of the 55 kDa protein did not show any homology to proteins in the databases. Proteomic analysis of these proteins leads to the conclusion that the sequence data provided the protein databases of the crystal proteins of the __konkukian__ ssp.
Who reads Mass spectrometric sequencing of endotoxin proteins of ** Bacillus thuringiensis ** ssp. ** konkukian ** extracted from polyacrylamide gels?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Kwang Yong Lee; Eun Young Kang; Sooil Park; Seong Kyu Ahn; Kwan Hee Yoo; Jin Young Kim; Hyung Hoan Lee
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2006
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)