Can I read Chemokine Receptors Are Expressed Widely by Embryonic and Adult Neural Progenitor Cells on EtoBox?

Chemokine Receptors Are Expressed Widely by Embryonic and Adult Neural Progenitor Cells by Phuong B. Tran; Dongjun Ren; Thomas J. Veldhouse; Richard J. Miller is a Neuroscience article available to read on EtoBox.

What is Chemokine Receptors Are Expressed Widely by Embryonic and Adult Neural Progenitor Cells about?

## Abstract We investigated the expression and functions of chemokine receptors in neural progenitor cells isolated from embryonic and adult mice. Reverse transcriptase‐polymerase chain reaction (RT‐PCR) analysis demonstrated mRNA expression for most known chemokine receptors in neural progenitor cells grown as neurospheres from embryonic (E17) and adult (4‐week‐old) mice. The expression of CXCR4 receptors was demonstrated further in E17 neurospheres using immunohistochemistry, in situ hybridization, Northern blot analysis and fura‐2‐based Ca^2+^ imaging. Most neurospheres grown from E17 mice responded to stromal cell‐derived factor‐1 (SDF‐1/CXCL12) in Ca^2+^ imaging studies. In addition, immunohistochemical studies demonstrated that these neurospheres consisted of dividing cells that uniformly colocalized nestin and CXCR4 receptors. Differentiation of E17 neurospheres yielded astrocytes and neurons exhibiting several different phenotypes, including expression of calbindin, calretinin, gamma‐aminobutyric acid (GABA), and glutamate, and many also coexpressed CXCR4 receptors. In addition, neurospheres grown from the subventricular zone (SVZ) of 4‐week‐old mice exhibited large increas

Who reads Chemokine Receptors Are Expressed Widely by Embryonic and Adult Neural Progenitor Cells?

Author

Phuong B. Tran; Dongjun Ren; Thomas J. Veldhouse; Richard J. Miller

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0360-4012)

Published

2004

Language

EN

Field

Neuroscience (Life Sciences)