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Fabrication, characterization, and biological assessment of multilayer DNA coatings on sandblasted-dual acid etched titanium surface by Li Liu; Li-Na Song; Guo-Li Yang; Shi-Fang Zhao; Fu-Ming He is a Engineering article available to read on EtoBox.

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## Abstract As local gene therapy has received attention, immobilizing functional gene onto irregular oral implant surface has become an advanced challenge. Electrostatic layer‐by‐layer (LBL) assembly technique could achieve this goal and allow local and efficient administration of genes to the target cells. In this study, multilayers of cationic lipid/plasmid DNA (pEGFP‐C1) complex (LDc) and anionic hyaluronic acid were assembled onto sandblasted‐dual acid etched titanium disks by the LBL technique. Surface characteristics of the coatings were performed by x‐ray photospectroscopy (XPS), contact angle measurements, and scanning electron microscopy (SEM). The cell biological characteristics of the coatings were evaluated by __in vitro__ experiments. SEM results demonstrated that the porous titanium surface was gradually flattened with the increase of the multilayer. The XPS survey indicated that the N element was found from the coating. The coating degradation and pEGFP‐C1 releasing kinetics showed that the more assembled layer numbers were, the larger the amount of DNA released in the first 30 h. MC3T3‐E1 cells were cultured directly on the DNA‐loaded surface. Higher enhanced green

Who reads Fabrication, characterization, and biological assessment of multilayer DNA coatings on sandblasted-dual acid etched titanium surface?

Author

Li Liu; Li-Na Song; Guo-Li Yang; Shi-Fang Zhao; Fu-Ming He

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1549-3296)

Published

2011

Language

EN

Field

Engineering (Physical Sciences)