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Phylogeography and host association in a pollinating seed parasite Greya politella (Lepidoptera: Prodoxidae) by J. M. Brown; J. H. Leebens-Mack; J. N. Thompson; O. Pellmyr; R. G. Harrison is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Phylogeography and host association in a pollinating seed parasite Greya politella (Lepidoptera: Prodoxidae) about?

Inferring the historical context of ecological diversification is an important step in understanding the way that population-level processes result in a diversity of species and interactions in communities. We performed a phylogeographic analysis of mitochondrial DNA haplotypes from the pollinating seed parasite Greya politella (Lepidoptera: Prodoxidae) in order to determine the degree to which populations were structured according to geographical location and host-plant association. Ninety-eight individuals were sampled from 29 locations ranging from southern California to western Idaho. Restriction-site variation in 87 individuals (27 populations) was screened by digestion with 11 endonucleases, followed by Southern blotting; 38 restriction-site positions were mapped by double digests. Haplotypes were further defined by generating fragments 251 bases in length via PCR, screening them for sequence variation using denaturing gel gradient electrophoresis (DGGE), and sequencing the resulting variants. Parsimony analysis of the resulting 12 restriction-site and 15 sequence haplotypes indicated strong geographical structuring of populations: (i) most populations were monomorphic for ha

Who reads Phylogeography and host association in a pollinating seed parasite Greya politella (Lepidoptera: Prodoxidae)?

Author

J. M. Brown; J. H. Leebens-Mack; J. N. Thompson; O. Pellmyr; R. G. Harrison

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley; Research Square (ISSN 0962-1083)

Published

1997

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)