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Can I read Purification of Tubulin with Controlled Post-translational Modifications by Polymerization–depolymerization Cycles on EtoBox?
Purification of Tubulin with Controlled Post-translational Modifications by Polymerization–depolymerization Cycles by Souphron, Judith; Bodakuntla, Satish; Jijumon, A. S.; Lakisic, Goran; Gautreau, Alexis M.; Janke, Carsten; Magiera, Maria M. is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Purification of Tubulin with Controlled Post-translational Modifications by Polymerization–depolymerization Cycles about?
In vitro reconstitutions of microtubule assemblies have provided essential mechanistic insights into the molecular bases of microtubule dynamics and their interactions with associated proteins. The tubulin code has emerged as a regulatory mechanism for microtubule functions, which suggests that tubulin isotypes and post-translational modifications (PTMs) play important roles in controlling microtubule functions. To investigate the tubulin code mechanism, it is essential to analyze different tubulin variants in vitro. Until now, this has been difficult, as most reconstitution experiments have used heavily post-translationally modified tubulin purified from brain tissue. Therefore, we developed a protocol that allows purification of tubulin with controlled PTMs from limited sources through cycles of polymerization and depolymerization. Although alternative protocols using affinity purification of tubulin also yield very pure tubulin, our protocol has the unique advantage of selecting for fully functional tubulin, as non-polymerizable tubulin is excluded in the successive polymerization cycles. It thus provides a novel procedure for obtaining tubulin with controlled PTMs for in vitro
Who reads Purification of Tubulin with Controlled Post-translational Modifications by Polymerization–depolymerization Cycles?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Souphron, Judith; Bodakuntla, Satish; Jijumon, A. S.; Lakisic, Goran; Gautreau, Alexis M.; Janke, Carsten; Magiera, Maria M.
- Publisher
- Nature Publishing Group; Springer Science and Business Media LLC; ScienceOpen (ISSN 1750-2799)
- Published
- 2019
- Language
- EN
- ISBN
- 9784159601905
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)