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Evaluation of active human herpesvirus 6 infection by reverse transcription‐PCR by Tetsushi Yoshikawa; Shiho Akimoto; Naoko Nishimura; Takao Ozaki; Masaru Ihira; Masahiro Ohashi; Masashi Morooka; Sadao Suga; Yoshizo Asano; Masaya Takemoto; Yukihiro Nishiyama is a Medicine article available to read on EtoBox.

What is Evaluation of active human herpesvirus 6 infection by reverse transcription‐PCR about?

## Abstract Monitoring of active human herpesvirus 6 (HHV‐6) infection is important for distinguishing between reactivation and latency of the virus. The reverse transcription polymerase chain reaction (RT‐PCR) may be a useful tool in order to distinguish active and latent HHV‐6 infection. An RT‐PCR assay detecting 4 different HHV‐6 gene transcripts was established. Samples of peripheral blood mononuclear cells (PBMCs) were collected from patients with exanthem subitum and used to evaluate the reliability of the assay. After confirming the reliability of the assay, RT‐PCR was used to determine whether HHV‐6 reactivation occurs in children with hypercytokinemia. Three gene transcripts (U31, U39, and U94) were detected in 90–100% of the PBMC samples collected from febrile period of exanthem subitum patients, from which HHV‐6 was isolated. The two gene transcripts encoding the late proteins U31 and U39, however, were not detected in samples collected during the convalescent period that contained no infectious virus. The putative latency associated gene transcript, U94, was detected in 2 (10%) of the 20 convalescent samples, and another immediate early gene transcript, U90, was also de

Who reads Evaluation of active human herpesvirus 6 infection by reverse transcription‐PCR?

Author

Tetsushi Yoshikawa; Shiho Akimoto; Naoko Nishimura; Takao Ozaki; Masaru Ihira; Masahiro Ohashi; Masashi Morooka; Sadao Suga; Yoshizo Asano; Masaya Takemoto; Yukihiro Nishiyama

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0146-6615)

Published

2003

Language

EN

Field

Medicine (Health Sciences)