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An approach to the structural analysis of oligosaccharides by N.M.R. spectroscopy by J.Howard Bradbury; J.Grant Collins is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is An approach to the structural analysis of oligosaccharides by N.M.R. spectroscopy about?
The proton chemical shifts of the glycosldlc protons have been measured for solutions of ohgosaccharldes m D,O, to some degree, the shifts are dlagnostlc of both the nature of the sugar and of the type of linkage To determme the sequence of the sugar residues, the aldose or ketose IS first treated with cyamde to produce a termmal carboxyhc acid Then Gd3+ 1s added, which IS bound on the carboxyl group, causmg hne broadening of the proton resonances and a decrease m the spin-Iattlce relaxation-time (T1) of the glycosldlc protons These effects fall off progressively along the ohgosaccharlde chain, hence allowing the sequence to be determined The method, &uch 1s illustrated for maltotnose, has been used at the mllhgram level, and may be apphcable up to pentasacchandes Dlfficultles occur m the treatment of nonreducmg sugars (I-1 and l-2 glycosldes), and with certam sugar residues that bmd Gd3+ on three adJaCent hydroxyl-groups of particular stereochemistry The possible usefulness of 13C-n m r spectroscopy IS also considered INTR(JXJCTION
Who reads An approach to the structural analysis of oligosaccharides by N.M.R. spectroscopy?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- J.Howard Bradbury; J.Grant Collins
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0008-6215)
- Published
- 1979
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)