Can I read Cancer Treatment by Photodynamic Therapy Combined with Adoptive Immunotherapy Using Genetically Altered Natural Killer Cell Line on EtoBox?

Cancer Treatment by Photodynamic Therapy Combined with Adoptive Immunotherapy Using Genetically Altered Natural Killer Cell Line by Mladen Korbelik; Jinghai Sun is a Medicine article available to read on EtoBox.

What is Cancer Treatment by Photodynamic Therapy Combined with Adoptive Immunotherapy Using Genetically Altered Natural Killer Cell Line about?

Adoptive immunotherapy mediated by human natural killer (NK) cell line genetically altered to produce interleukin-2 (NK92MI) was evaluated as adjuvant to photodynamic therapy (PDT) of subcutaneous tumors. The combined effect of these two modalities was first examined with SiHa tumors (human cervical squamous cell carcinoma) growing in NOD-scid mice. The most effective protocol for NK92MI cell transfer in conjunction with PDT mediated by photosensitizer mTHPC was the injection of 5 x 10(7) cells (peritumoral or intravenous) given immediately after PDT, which produced a marked improvement in the therapeutic outcome compared with the effect of PDT alone. The same protocol was tested with HT-29 tumor model (human colorectal adenocarcinoma) xenografted in NOD-scid mice. The results demonstrate that the adoptive immunotherapy with NK92MI cells (which when used alone were not effective in controlling tumor growth) significantly improved the cures of PDT-treated HT-29 tumors, whereas such benefit was not observed with the parental cell line NK92 (not producing interleukin-2). Flow cytometry-based analysis revealed a higher percentage of p.t. injected NK92MI cells in PDT-treated than in non

Who reads Cancer Treatment by Photodynamic Therapy Combined with Adoptive Immunotherapy Using Genetically Altered Natural Killer Cell Line?

Author

Mladen Korbelik; Jinghai Sun

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

2001

Language

EN

Field

Medicine (Health Sciences)