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Study of Bradykinin Metabolism in Human and Rat Plasma by Liquid Chromatography with Inductively Coupled Plasma Mass Spectrometry and Orthogonal Acceleration Time-of-flight Mass Spectrometry by Peter Marshall; Olivier Heudi; Stephen Mckeown; Augustin Amour; Fadi Abou-Shakra is a Chemistry article available to read on EtoBox.

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## Abstract Bradykinin is a small peptide that acts mainly as a hormone by activating specific receptors that confer protection against the development of hypertension. The efficacy of bradykinin is influenced by the activities of various kininases present in plasma and blood. In this study, both human and rat plasma were incubated with a labelled form of bradykinin (at 4 and 12.5 μM), that will be referred to as bromobradykinin. The metabolic fate of bromobradykinin was monitored by liquid chromatography coupled to an orthogonal acceleration time‐of‐flight mass spectrometer (oaTOF). Quantification measurements of the bromine‐containing metabolites were performed on‐line, via flow splitting, by inductively coupled plasma mass spectrometry (ICPMS). The data obtained highlighted that the mechanism(s) of bradykinin metabolism in human and rat plasma are different, with the metabolism of bradykinin in rat plasma being much more aggressive than that observed in human plasma. In addition to the known bradykinin metabolites, e.g. [1,5], [1,7] from ACE, [1,8] from carboxypeptidase and [2,9] from aminopeptidase activity, we have identified the presence of new bradykinin metabolites in both

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Author

Peter Marshall; Olivier Heudi; Stephen Mckeown; Augustin Amour; Fadi Abou-Shakra

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)

Published

2002

Language

EN

Field

Chemistry (Physical Sciences)