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A fibrinogen-related Lectin from Echinometra lucunter represents a new FReP family in Echinodermata phylum by Philippe Lima Duarte; Francisco Regivânio Nascimento Andrade; Andressa Rocha de Oliveira Sousa; Alexandre Lopes Andrade; Mayron Alves de Vasconcelos; Edson Holanda Teixeira; Celso Shiniti Nagano; Alexandre Holanda Sampaio; Rômulo Farias Carneiro is a Immunology and Microbiology article available to read on EtoBox.
What is A fibrinogen-related Lectin from Echinometra lucunter represents a new FReP family in Echinodermata phylum about?
Fibrinogen-related proteins (FREPs) have been identified in several animals. They are involved in the body's defense, acting as mediators of phagocytosis. Ficolins and intelectins are some of the most studied Fibrinogen-related Domain (FReD)-containing lectins. In this work, we have isolated a singular FReD-containing lectin, which cannot be classified as ficolin or intelectin. ELL (Echinometra lucunter lectin) was isolated from coelomic plasma by affinity chromatography on xanthan gum. Primary structure was determined by tandem mass spectrometry. Moreover, antimicrobial activity of ELL was evaluated against planktonic cells and biofilm of Escherichia coli, Staphylococcus aureus and S. epidermidis. ELL showed hemagglutinating activity in Ca presence, which was inhibited by glycoprotein mucin and thyroglobulin. Complete amino acid sequence consisted of 229 residues, including a FReD in the N-terminal. Searches for similarity found that ELL was very close to putative proteins from Strongylocentrotus purpuratus. ELL showed moderate similarity with uncharacterized sea stars proteins and protochordate intelectins. ELL was able to inhibit the planktonic growth of the Gram-positive bacter
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- Author
- Philippe Lima Duarte; Francisco Regivânio Nascimento Andrade; Andressa Rocha de Oliveira Sousa; Alexandre Lopes Andrade; Mayron Alves de Vasconcelos; Edson Holanda Teixeira; Celso Shiniti Nagano; Alexandre Holanda Sampaio; Rômulo Farias Carneiro
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Immunology and Microbiology (Life Sciences)