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Structure of the fission yeast centromere cen3: Direct analysis of the reiterated inverted region by Shin Murakami; Tomohiro Matsumoto; Osami Niwa; Mitsuhiro Yanagida is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Structure of the fission yeast centromere cen3: Direct analysis of the reiterated inverted region about?

We determined the structure of the Schizosaccharomyces pombe centromere cen3 using direct genomic mapping and cosmid walking. The repetitive region of cen3 is approximately 110 kb, much longer than that of the previously determined cenl and cen2 regions. The 30 kb long left and ~ 60 kb right repetitive sequences are arranged with an inverted symmetry and flank the 15~20 kb central domain. The repeat motifs in cen3, although they consist of the common centromeric repeat elements, are slightly different from those in cenl and cen2. The ten3 repeat motifs appear to be reiterated four times in the left and nine times in the right side repetitive regions. We found that the central domain consists of the common ~ 5 kb core sequence associated with the pair of innermost inverted sequences, most of which are reiterated only twice in the genome. Although their sizes differ significantly, the general features of cent, cen2 and cen3 are similar, and a prototype, consensus structure for the fission yeast centromere may be deduced.

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Author

Shin Murakami; Tomohiro Matsumoto; Osami Niwa; Mitsuhiro Yanagida

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0009-5915)

Published

1991

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)