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Temporal Changes in Gene Expression Induced by Sulforaphane in Human Prostate Cancer Cells by Suvarna Bhamre; Debashis Sahoo; Robert Tibshirani; David L. Dill; James D. Brooks is a Medicine article available to read on EtoBox.

What is Temporal Changes in Gene Expression Induced by Sulforaphane in Human Prostate Cancer Cells about?

## Abstract ## BACKGROUND Prostate cancer is thought to arise as a result of oxidative stresses and induction of antioxidant electrophile defense (phase 2) enzymes has been proposed as a prostate cancer prevention strategy. The isothiocyanate sulforaphane, derived from cruciferous vegetables like broccoli, potently induces surrogate markers of phase 2 enzyme activity in prostate cells in vitro and in vivo. To better understand the temporal effects of sulforaphane and broccoli sprouts on gene expression in prostate cells, we carried out comprehensive transcriptome analysis using cDNA microarrays. ## METHODS Transcripts significantly modulated by sulforaphane over time were identified using StepMiner analysis. Ingenuity Pathway Analysis (IPA) was used to identify biological pathways, networks, and functions significantly altered by sulforaphane treatment. ## RESULTS StepMiner and IPA revealed significant changes in many transcripts associated with cell growth and cell cycle, as well as a significant number associated with cellular response to oxidative damage and stress. Comparison to an existing dataset suggested that sulforaphane blocked cell growth by inducing G2/M arrest. Cell gr

Who reads Temporal Changes in Gene Expression Induced by Sulforaphane in Human Prostate Cancer Cells?

Author

Suvarna Bhamre; Debashis Sahoo; Robert Tibshirani; David L. Dill; James D. Brooks

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0270-4137)

Published

2008

Language

EN

Field

Medicine (Health Sciences)