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Quantification of in Vitro Mineralisation Using Ion Chromatography by Paul Souter; Alan Horner; Jim C. Cunningham is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Analysis of in vitro mineralisation is an important tool in orthopedic research, allowing assessment of new therapeutic agents and devices; however, access to analytical equipment and accuracy of current methods can be a limiting factor. This current work investigated the use of calcium chelation with citric acid and subsequent analysis by ion chromatography as a method for accurately quantifying the extent of in vitro calcium deposition. Primary human osteoblasts were cultured on tissue culture plastic for 21 days under osteogenic conditions. At 3, 7, 14, and 21 days, alizarin red staining and citric acid calcium chelation of the cultures were performed. The use of alizarin red revealed increased calcium deposition over the culture period but was not sensitive enough to detect mineralisation at early time points after taking in to account background residual staining. The use of ion chromatography gave a limit of detection of 2 lg calcium, sensitive enough to detect mineralisation after 3 days, with no issues relating to background levels. We believe that the use of ion chromatography for quantifying in vitro mineralisation gives researchers an accurate, accessible, and cheap way

Who reads Quantification of in Vitro Mineralisation Using Ion Chromatography?

Author

Paul Souter; Alan Horner; Jim C. Cunningham

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

2011

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)