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Evaluation of Secondary Ions Related to Plant Tissue Using Least Absolute Shrinkage and Selection Operator by Ito, Masaru; Kuga, Yukari; Yamagishi, Takayuki; Fujita, Miya; Aoyagi, Satoka is a Engineering article available to read on EtoBox.

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With regard to life sciences, it is important to understand biological functions such as metabolic reactions at the cellular level. Time-of-flight secondary ion mass spectrometry (TOF-SIMS) that can provide chemical mappings at 100 nm lateral resolutions is useful for obtaining three-dimensional maps of biological molecules in cells and tissues. TOF-SIMS spectra generally contain several hundred to several thousand secondary ion peaks that provide detailed chemical information. In order to manage such a large number of peaks, data analysis methods such as multivariate analysis techniques have been applied to TOF-SIMS data of complex samples. However, the interpretation of the data analysis results is sometimes still difficult, especially for biological samples. In this study, TOF-SIMS data of resin-embedded plant samples were analyzed using one of the sparse modeling methods, least absolute shrinkage and selection operator (LASSO), to directly select secondary ions related to biological structures such as cell walls and nuclei. The same sample was measured by optical microscopy and the same measurement area as TOF-SIMS was extracted in order to prepare a target image for LASSO. The

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Author

Ito, Masaru; Kuga, Yukari; Yamagishi, Takayuki; Fujita, Miya; Aoyagi, Satoka

Publisher

Springer-Verlag; American Institute of Physics; American Vacuum Society; AIP Publishing; Springer Science and Business Media LLC (ISSN 1934-8630)

Published

2020

Language

EN

Field

Engineering (Physical Sciences)