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Incorporation of Basic Fibroblast Growth Factor by a Layer-by-layer Assembly Technique to Produce Bioactive Substrates by Lie Ma; Jie Zhou; Changyou Gao; Jiacong Shen is a Medicine article available to read on EtoBox.

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## Abstract Basic fibroblast growth factor (bFGF) was immobilized onto quartz slides and collagen films by assembly with chondroitin sulfate (CS) in a layer‐by‐layer (LBL) manner. First, the LBL‐deposition process on the amino‐silanized quartz slides was monitored by UV–vis spectroscopy and water contact angle measurement. By substituting the normal bFGF with rhodamine‐labeled one (Rd‐bFGF), a linear increase of the absorbance versus bilayer number was recorded. The water contact angle oscillated between the odd CS and the even bFGF layers, demonstrating the alternating change of the surface chemistry. Scanning force microscopy (SFM) revealed that the surface topography was altered slightly after multilayer assembly. __In vitro__ incubation of the CS/bFGF multilayers in PBS showed that ∼30% of the incorporated bFGF was released within 8 days. __In vitro__ cell culture found that the fibroblasts showed star‐like morphology with plenty of pseudopods on the bFGF‐incorporated collagen film after cultured for 1 day, and the collagen films assembled with bFGF possess improved bioactivity than that of the virgin one and the bFGF control. Since the immobilized growth factors can maximally

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Author

Lie Ma; Jie Zhou; Changyou Gao; Jiacong Shen

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1552-4973)

Published

2007

Language

EN

Field

Medicine (Health Sciences)