Opening book details…
Can I read A proteomic analysis of C-reactive protein stimulated THP-1 monocytes on EtoBox?
A proteomic analysis of C-reactive protein stimulated THP-1 monocytes by Steffen U Eisenhardt; Jonathon Habersberger; Karen Oliva; Graeme I Lancaster; Mustafa Ayhan; Kevin J Woollard; Holger Bannasch; Greg E Rice; Karlheinz Peter is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is A proteomic analysis of C-reactive protein stimulated THP-1 monocytes about?
## Background C-reactive protein (CRP) is a predictor of cardiovascular risk. It circulates as a pentameric protein in plasma. Recently, a potential dissociation mechanism from the disc-shaped pentameric CRP (pCRP) into single monomers (monomeric or mCRP) has been described. It has been shown that mCRP has strong pro-inflammatory effects on monocytes. To further define the role of mCRP in determining monocyte phenotype, the effects of CRP isoforms on THP-1 protein expression profiles were determined. The hypothesis to be tested was that mCRP induces specific changes in the protein expression profile of THP-1 cells that differ from that of pCRP. ## Methods Protein cell lysates from control and mCRP, pCRP or LPS-treated THP-1 cells were displayed using 2-dimensional SDS PAGE and compared. Differentially expressed proteins were identified by MALDI-TOF MS and confirmed by Western blotting. ## Results mCRP significantly up-regulates ubiquitin-activating enzyme E1, a member of the ubiquitin-proteasome system in THP-1 monocytes. Furthermore, HSP 70, alpha-actinin-4 (ACTN4) and alpha-enolase/enolase 1 were upregulated. The proteomic profile of LPS and pCRP treated monocytes differ signific
Who reads A proteomic analysis of C-reactive protein stimulated THP-1 monocytes?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Steffen U Eisenhardt; Jonathon Habersberger; Karen Oliva; Graeme I Lancaster; Mustafa Ayhan; Kevin J Woollard; Holger Bannasch; Greg E Rice; Karlheinz Peter
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central, c2003-; Springer Science and Business Media LLC (ISSN 1477-5956)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)