Can I read Extraordinary Enantiospecificity of Lipase Catalysis in Organic Media Induced by Purification and Catalyst Engineering on EtoBox?

Extraordinary Enantiospecificity of Lipase Catalysis in Organic Media Induced by Purification and Catalyst Engineering by Shau-Wei Tsai; Jonathan S. Dordick is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Extraordinary Enantiospecificity of Lipase Catalysis in Organic Media Induced by Purification and Catalyst Engineering about?

A purified lipase preparation from Candida rugosa was compared t o its crude counterpart in anhydrous and slightly hydrated hydrophobic organic solvents. The purified lipase preparation was less active than the crude enzyme in dry n-heptane, whereas the presence of small concentrations of added water dramatically activated the purified enzyme but not the crude enzyme. Thus, in the presence of as little as 0.25 pUmL of added water in n-heptane, the purified enzyme is over 230-fold more active and 6-fold more enantioselective than the dry enzyme suspension in the esterification of racemic 2-(4-ch1orophenoxy)propionic acid with n-butanol. The reactivity and selectivity of this biocatalyst, however, was affected by coalescence of the enzyme preparation suspended in the wet organic solvent. Engineering the biocatalyst environment by dissolving the purified lipase in aqueous buffer and then adding this solution to n-heptane resulted in a precipitated enzyme preparation with smaller particle sizes that did not coalesce severely. In the presence of 5 pUmL of water added with the enzyme, this pretreatment resulted in an activation over the dry, purified enzyme preparation of over 1800-fold

Who reads Extraordinary Enantiospecificity of Lipase Catalysis in Organic Media Induced by Purification and Catalyst Engineering?

Author

Shau-Wei Tsai; Jonathan S. Dordick

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

2000

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)