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Developmental regulation of neurogenesis in the pluripotent human embryonal carcinoma cell line NTERA-2 by S. A. Przyborski; I. E. Morton; A. Wood; P. W. Andrews is a Neuroscience article available to read on EtoBox.

What is Developmental regulation of neurogenesis in the pluripotent human embryonal carcinoma cell line NTERA-2 about?

## Abstract Embryonal carcinoma (EC) cells provide a caricature of pluripotent embryonic stem (ES) cells and may be used as surrogates for investigating the mechanisms that regulate cell differentiation during embryonic development. NTERA‐2 is a human EC cell line that differentiates in response to retinoic acid yielding cells that include terminally differentiated neurons. The expression of genes known to be involved in the formation of the vertebrate nervous system was examined during retinoic acid‐induced NTERA‐2 differentiation. Differentiation of these human EC cells into neurons could be divided into three sequential phases. During phase 1, in the first week of differentiation, hath1 mRNA showed a small transient increase that correlated with the rapid accumulation of nestin message, a marker of neuroprogenitors. Transcripts of nestin were quickly downregulated during phase 2 as expression of neuroD1, characteristic of neuroprogenitors exiting the cell cycle, was induced. A neural cell surface antigen, detected by the monoclonal antibody A2B5, was expressed by cells exiting the cell cycle, correlating with the expression of neuroD1 as the cells became post‐mitotic. Markers of

Who reads Developmental regulation of neurogenesis in the pluripotent human embryonal carcinoma cell line NTERA-2?

Author

S. A. Przyborski; I. E. Morton; A. Wood; P. W. Andrews

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0953-816X)

Published

2000

Language

EN

Field

Neuroscience (Life Sciences)